Analysis of competition in soil among 2,4-dichlorophenoxyacetic acid-degrading bacteria
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چکیده
منابع مشابه
Analysis of competition in soil among 2,4-dichlorophenoxyacetic acid-degrading bacteria.
Competition among indigenous and inoculated 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria was studied in a native Kansas prairie soil following 2,4-D additions. The soil was inoculated with four different 2,4-D-degrading strains at densities of 10(3) cells per g of soil; the organisms used were Pseudomonas cepacia DBO1(pJP4) and three Michigan soil isolates, strain 745, Sphingomonas...
متن کامل2,4-Dichlorophenoxyacetic acid-degrading bacteria contain mosaics of catabolic genes.
DNA from 32 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria from diverse locations was probed with the first three genes of the well-known 2,4-D degradation pathway found in Alcaligenes eutrophus JMP134(pJP4). The majority of strains did not show high levels of homology to the first three genes of the 2,4-D degradation pathway, tfdA, -B, and -C. Most strains showed combinations of tfd...
متن کاملIdentification of in situ 2,4-dichlorophenoxyacetic acid-degrading soil microorganisms using DNA-stable isotope probing
Stable isotope probing (SIP) was used to investigate the microorganisms responsible for degradation of the herbicide, 2,4dichlorophenoxyacetic acid (2,4-D) in soil samples. Soils were unamended or amended with either unlabeled 2,4-D or UL(ring) C-2,4D. Degradation of 2,4-D was complete after 17 days, whereas little removal (1173%) was observed in the sterile controls. Terminal restriction fragm...
متن کاملPartial Purification and Characterization of 2,4- Dichlorophenoxyacetic Acid Degrading Bacteria Harboring Alpha Ketoglutarate Dioxgenase Enzyme
The catabolic adaptability demonstrated by microorganisms is important in the bioremediation of polluted environments. 2,4-dichlorophenoxyacetic acid/α-ketoglutarate dioxygenase is the first enzyme in the catabolic pathway for the 2,4dichlorophenoxyacetic acid (2,4-D). In the present study, dioxygenase enzymes were inducible and the serial adaptation of four studied bacterial isolates steadily ...
متن کاملUse of gene probes to aid in recovery and identification of functionally dominant 2,4-dichlorophenoxyacetic acid-degrading populations in soil.
The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) was applied to soils in microcosms, and degradation was monitored after each of five repeated additions. Total DNAs were isolated from soil bacterial communities after each 2,4-D treatment. The DNA samples were analyzed on slot blots and Southern blots by using a tfdA gene probe subcloned from plasmid pJP4 and a Spa probe derived from a diffe...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1994
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.60.4.1121-1128.1994